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Title: In Vitro Propagation of Sweet Potato (Ipomoea Batatas (L.) Lam.) Through Lateral Bud Culture
Authors: Berihu Mengs
Meseret Chimdessa
Keywords: Auxin, Cytokinin, Growth regulators, Kullufo, MS medium, Tulla,
Issue Date: Jun-2014
Publisher: Haramaya University
Abstract: Sweet potato is an economically important vegetable and root crop that belongs to the family Convolvulaceae. The world sweet potato demand for the use as vegetative and root crop is consistently increasing. However, the production of sweet potato has been generally low mainly due to a multitude of biotic and a biotic stresses. Thus, an experiment was conducted with the aim of developing an in vitro propagation protocol for sweet potato cultivation in a completely randomized design (CRD) in three replications. For callus inductions, lateral bud of two varieties, Kullufo and Tulla were cultured on MS basal medium that contained 30 g/l sucrose, 7 g/l agar, and vitamins supplemented with 1, 2, 3 and 4 mg/l of NAA combined with 2 mg/l of Kn. In shoot multiplication, MS media supplemented with 0.5, 1, 1.5 and 2 mg/l of BAP or 0.5, 1, 1.5 and 2 mg/l of Kn, and combinations of the two PGRs in a ratio of 0.5 mg/l BAP: 2 mg/l of Kn or 2 mg/l BAP: 0.5 mg/l of Kn were used. In root inductions, MS media supplemented with 0.25, 0.5, 0.75 and 1 mg/l of IBA or 0.25, 0.5, 0.75 and 1 mg/l of NAA, and combinations of the two PGRs in a ratio of 0.25 mg/l of IBA: 1 mg/l of NAA or 1 mg/l of IBA: 0.25mg/l of NAA were used. In acclimatization, the effect of PGRs and four different culture substrates on the survival of in vitro propagated sweet potato were studied. The results showed that the best callus formation, 97.7 & 95.5%, was observed when MS medium was supplemented with 2 mg/l NAA in combination with 2 mg/l of kinetin. In shoot formation experiment, best shooting was observed at 0.5 and 1 mg/l concentrations of both cytokinins (BAP and Kn). Moreover, a combination of the two PGRs in a ratio of 0.5 mg/l of BAP with 2 mg/l of Kn resulted better shooting than their combination in a ratio of 2mg/l of BAP with 0.5 mg/l of Kn. The two PGRs used for rooting (IBA and NAA) had significant rooting effect at 0.25 and 0.5 mg/l than at 0.75mg/l and 1mg/l. The mixture of red soil, sand soil and compost (1:2:1) cultural substrates showed 81.25% and 70.59% plantlet survival for Kullufo and Tulla sweet potato varieties, respectively. In conclusion, this study provided optimal protocol for micro-propagation of sweet potato varieties through lateral bud culturing on MS basal medium supplemented with appropriate concentrations of different PGRs in sole or combination. This protocol can thus be utilized to micro-propagate the two sweet potato varieties to boost its production. However, more refinement of the protocol is subject to further analysis. Further studies must also be considered with different strengths of MS media and other plant growth regulators such as TDZ, 2, 4D and 2ip for callus induction and shoot regeneration.
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